S a f e t y     D a t a     S h e e t

According to Regulation (EC) 1907/2006

2053  Calcein 


1.      Identification of the substance/preparation and of the company or firm

1.1  Identification of the substance or preparation





bis-[N,N-bis-(Carboximethyl)Aminomethyl] Fluorescein, Fluorescein Complexone, Fluorexone


REACH Registration Number: A registration number is not available for this substance as the substance or its use are exempted from registration according to Article 2 REACH Regulation (EC) Nº 1907/2006, the annual tonnage does not requiere a registration, the registration is envisaged for a later registration deadline or it is a mixture.


1.2  Use of the substance/preparation:

For laboratory utilisation, analysis, research and fine chemistry.


1.3  Identification of the company or firm:


C/Garraf 2

Polígono Pla de la Bruguera

E-08211 Castellar del Vallès

(Barcelona)  Spain

Tel. (+34) 937 489 400

e-mail: product.safety@panreac.com


1.4  Emergency telephone:

Single telephone number for emergency calls: 112 (EU)

Tel.: (+34) 937 489 499


2.      Identification of dangers


Classification of the substance or the mixture.




No hazardous substance as specified in Regulation (CE) 1272/2008.



No hazardous substance as specified in Classification (67/548/CEE or 1999/45/CE).


3.       Component Composition/Information



Name: Calcein

Formula: C30H26N2O13        M.=  622,54    CAS [1461-15-0]

EC number (EINECS): 215-957-1



4.       First aid


4.1  General indications:

Never provide drink or induce vomiting in the event of loss of consciousness.


4.2  Inhaling:

Take the person out into the fresh air.


4.3  Contact with the skin:

Wash with plenty of water. Remove contaminated clothing.


4.4  Eyes:

Wash with plenty of water, keeping eyelids open.


4.5  Swallowing:

Drink large amounts of water. Induce vomiting. Seek medical assistance.


5.      Fire-fighting means


5.1  Suitable fire-extinguishing means:

Water. Carbon dioxide (CO2). Foam. Dry powder.


5.2  Fire-fighting means which must NOT be used:

No specific data.


5.3  Special risks:



5.4  Protective equipment:

Suitable clothing and footwear.


6.       Measures to be taken in the event of accidental spillage


6.1  Individual precautions:

No special indications.


6.2  Precautions for care of the environment:

Avoid pollution of the soil, water supplies and drains.


6.3  Methods for collection/cleaning:

Collect up dry and deposit in waste containers for subsequent elimination in accordance with current legislation. Clean any remains with plenty of water.


7.       Handling and storage


7.1 Handling:

Handle under an extractor fan.


7.2 Storage:

 Well sealed containers.


8.       Staff exposure/protection controls


8.1  Technical protective measures:

No special indications.


8.2 Exposure limit control:

:  Data not available.



8.3 Respiratory protection:

If dust forms, use suitable respiratory protection.


8.4 Hand protection:

Use suitable gloves


8.5 Eye protection:

Use suitable goggles.


8.6 Individual hygiene measures:

Remove contaminated clothing. Wash hands before breaks and when the job is done. Do not inhale the substance.


8.7 Environmental exposure controls:

Fulfill the commitments under local environmental protection legislation.


9.      Physical and chemical properties


 Appearance: solid

Colour: Orange

Granulometry: N/A

Odour: Characteristic.



Melting point/freezing point: 300 °C

Initial boiling point and boiling range:


Flash point:


Flammability (solid, gas):


Upper/lower flammability or explosive limits:


Vapour pressure: N/A

Vapour density: N/A

Relative density:


Solubility: 4 g/l in water0,6 g/l in alcohol

Partition coefficient: n-octanol/water:


Auto-ignition temperature:


Decomposition temperature: N/A

Viscosity: N/A


10.  Stability and reactivity


10.1  Conditions which should be avoided: 

The product is chemically stable under standar ambient conditions (room temperature).  


10.2   Matter which should be avoided:

No specific data.


10.3  Hazardous decomposition products:

 No specific data.


10.4  Complementary information:

No specific data. 


11.  Toxicological information


11.1          Acute toxicity:

    :     Data not available.


11.2          Dangerous effects for health:

No specific data on this product known with regard to effects of overdose in humans. Other dangerous characteristics are not discarded. Take the usual precautions for handling chemical products.


12.  Environmental information


12.1  Toxicity:

12.1.1 - EC50 test (mg/l): 

12.1.2. - Receptor medium: 

Risk for the water environment 

Risk for the land environment 

12.1.3. - Observations: 


12.2  Persistence and Degradability :

12.2.1 - Test:


12.2.2. - Biotic degradation classification:



12.2.3. - Abiotic degradation depending on pH:

12.2.4. - Observations:


12.3  Bioaccumulative potential:

12.3.1. - Test:

12.3.2. - Bioaccumulation:


12.3.3. - Observations:


12.4  Mobility in soil :

Data not available.


12.5 Assessment PBT and MPMB :

Data not available.


12.6  Other possible effects on the environment:

If suitable handling conditions are maintained, no ecological problems are to be anticipated.



13. Considerations regarding elimination


13.1  Substance or preparation:

In the European Union, there are no homogeneous standards established for elimination of chemical waste, which is waste of a special nature, and treatment and elimination of same is subject to the domestic legislation in each country.

 In view of this, in each case, you should contact the competent authority or those companies legally authorized for elimination of waste.

2001/573/EC: Council Decision of 23 July 2001 amending Commission Decision 2000/532/EC as regards the list of wastes.Council Directive 91/156/EEC of 18 March 1991 amending Directive 75/442/EEC on waste.



13.2  Contaminated containers:

Contaminated containers and packaging of dangerous substances or preparations must be treated in the same manner as the actual products contained in them.

European Parliament and Council Directive 94/62/EC of 20 December 1994 on packaging and packaging waste.



14.  Information concerning transport


Not classified as dangerous in the meaning of transport regulations.


15.  Regulatory information


This safety datasheet complies with the requirements of Regulation (EC) No. 1907/2006.



16.  Other information


Review number and date: 4 15.09.2011

Date published: 15.09.2011

In respect of the previous review, changes have been made to the following sections: 15

The information included in this Safety Data Sheet is based on our most up-to-date knowledge, and is solely intended to inform regarding aspects of safety; the properties and characteristics indicated herein are not guaranteed.